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| 产品名称： | LLC-PK1 |
|---|---|
| 商品货号： | TS260013 |
| <strong>Cell line </strong>： | LLC-PK1 |
| DSMZ no. ： | TS260013 |
| Species ： | originally confirmed as pig by species PCR and recently as sus scrofa origin by COI DNA barcoding |
| Cell type ： | kidney |
| Origin ： | established in 1958 from the normal kidney of a juvenile male Hampshire pig (Sus scrofa); cells were described in the literature to exhibit properties characteristic of kidney proximal tubular epithelium |
| Reference(s) ： | 14599, 14622, 14845 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid cells growing adherently in monolayers |
| Medium ： | 90% Dulbecco´s MEM + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2; optimal split ratio of 1:5 to 1:10 every 3-4 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 24 hours |
| Harvest ： | cell harvest of ca. 15 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Viruses ： | SMRV - |