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| 产品名称： | DT-40 |
|---|---|
| 商品货号： | TS260014 |
| <strong>Cell line </strong>： | DT-40 |
| DSMZ no. ： | TS260014 |
| Species ： | confirmed as chicken by species PCR and thereafter as Gallus spec. Origin by COI DNA Barcoding |
| Cell type ： | B cell line |
| Origin ： | established in 1985 from a bursal lymphoma, which was induced by infection of a 1 day old chicken with Rous-associated virus type 1 (RAV-1), an avian leukosis retrovirus carrying no cell-derived oncogene; cells are described in the literature to retain the ability to rearrange the immunoglobulin light chain gene (IgL) and to exhibit a high frequency of homologous gene targeting |
| Reference(s) ： | 14367, 14411, 14412, 15187, 15186 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing in clumps of 10 to 100 cells in suspension |
| Medium ： | 80% Advanced RPMI 1640 + 20% h.i. FBS + 1 mM sodium pyruvate + 2 mM L-glutamine + 4.5 g/L glucose + 50 µM mercaptoethanol |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; optimal split ratio of 1:2 to 1:3 every 2-3 days, maintain at 0.5-1.5 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 48 hours |
| Harvest ： | maximum density at ca. 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Viruses ： | SMRV - |