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| 产品名称： | U-2940 |
|---|---|
| 商品货号： | TS260015 |
| <strong>Cell line </strong>： | U-2940 |
| DSMZ no. ： | TS260015 |
| Species ： | confirmed as human by species PCR |
| Cell type ： | B cell lymphoma |
| Origin ： | established in 1991 from the pleural effusion of a 18-year-old woman with diffuse large B-cell lymphoma (DLBCL) after being diagnosed earlier with Hodgkin lymphoma and treated with chemotherapy; recent molecular biological characterization assigned this cell line to the entity "primary mediastinal B-cell lymphoma" (PMBL); cells were described in the literature to bear microsatellite instability. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14917, 14917, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | small round cells growing singly and in clusters in suspension; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at 0.5-1.5 x 106 cells/ml; cells need 3-5 days to recover after thawing; it might be of advantage to start culture initially in a 12- or 24-well-plate |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | cell harvest of ca. 2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, cyCD79a +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG +, sm/cyIgM -, sm/cykappa +, cm/cylambda -; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human hypodiploid karyotype with 8% polyploidy; 45(43-45)X, -X, del(3)(p14p21), del(6)(q13q15), der(7)t(2;7)(q22;p22), dup(12)(q13q22), der(14)t(X;14)(q12;p11), t(16;16)(p12;p13), del(17)(p13); carries homozygous microdeletion of SOCS1 locus; resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |