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| 产品名称： | NT-92 |
|---|---|
| 商品货号： | TS260018 |
| <strong>Cell line </strong>： | NT-92 |
| DSMZ no. ： | TS260018 |
| Species ： | confirmed as rat by species PCR |
| Cell type ： | cholangio-hepatocellular tumor |
| Origin ： | established ca. 1986 from the cholangio-hepatocellular tumor induced in vivo by treatment of a male Wistar AF-Han rat with a single injection of N-methyl-N-nitrosourea and oral administration of phenobarbital |
| Reference(s) ： | 15145 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid cells growing adherently in monolayers |
| Medium ： | 90% MEM (with Earles salts) + 10% h.i. FBS |
| Subculture ： | seed out at ca. 2 x 106 cells/80 cm2; split confluent culture 1:2 to 1:4 every 4 days using trypsin/EDTA for 10 min |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 3-4 days |
| Harvest ： | cell harvest of ca. 15 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Viruses ： | SMRV - |