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| 产品名称： | MB-1 |
|---|---|
| 商品货号： | TS260022 |
| <strong>Cell line </strong>： | MB-1 |
| DSMZ no. ： | TS260022 |
| Species ： | confirmed as human by STR-typing |
| Cell type ： | anaplastic thyroid carcinoma |
| Origin ： | established in 2007 from the large retrosternal mass resected before treatment from a 57-year-old Caucasian man with rapidly fatal anaplastic thyroid cancer |
| Reference(s) ： | 14984 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round epitheloid cells growing in monolayers; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS + 2 mM L-glutamine |
| Subculture ： | seed out at ca. 1-3 x 106 cells/175 cm2; optimal split ratio of 1:3 to 1:10 every 3-7 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 60-100 hours |
| Harvest ： | cell harvest of ca. 10-15 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |