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| 产品名称： | ST |
|---|---|
| 商品货号： | TS260026 |
| <strong>Cell line </strong>： | ST |
| DSMZ no. ： | TS260026 |
| Species ： | originally confirmed as pig by species PCR and recently as sus scrofa origin by COI DNA barcoding |
| Cell type ： | testis |
| Origin ： | established from trypsinized testicular tissue of a porcine fetus |
| Reference(s) ： | 14755 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | fibroblastoid cells growing in monolayers |
| Medium ： | 90% MEM (with Earles salts) + 10% h.i. FBS + 1 mM sodium pyruvate + non-essential amino acids |
| Subculture ： | seed out at ca. 1 x 106 cells/80 cm2; split confluent culture 1:5 - 1:10 every 3-4 days using trypsin/EDTA (for more than 10 minutes) |
| Incubation ： | at 37 °C without CO2 (keep lid of flask closed) |
| Doubling time ： | ca. 30-40 hours |
| Harvest ： | cell harvest of ca. 4-5 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Viruses ： | SMRV - |