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| 产品名称： | DOGUM |
|---|---|
| 商品货号： | TS260029 |
| <strong>Cell line </strong>： | DOGUM |
| DSMZ no. ： | TS260029 |
| Species ： | confirmed as human by STR-typing |
| Cell type ： | Burkitt lymphoma |
| Origin ： | established in 2003 from the pleural fluid of a 49-year-old woman with B-cell non-Hodgkin lymphoma (transformed follicular centroblastic) after failed combination chemotherapy and radiation |
| Reference(s) ： | 14404 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | lymphoid cells growing in large clumps in suspension; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 0.3-0.5 x 106 cells/ml in 24-well-plates; maintain at ca. 0.7-1.5 x 106 cells/ml; optimal split ratio of 1:2 to 1:4 every 2-5 days; after thawing cells may need a few days to recover |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours; but up to 100 hours after recovery from N2 |
| Harvest ： | maximum densitiy of ca. 4.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD37 +, CD80 +, HLA-DR +, sm/cyIgG +, sm/cyIgM -, sm/cykappa -, sm/cylambda +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | highly rearranged human hypodiploid karyotype with 6% polyploidy; 43(40-45)<2n>X, -X, -14, -15, der(1)t(1;14)(q32;q21.2), t(14;18)(q32;q21), t(2;8)(q14;q24), der(2)t(2;8), dup(4)(p12p15), add(5)(q13), add(6)(p23), der(6;17)(p1?0;q1?0), add(10)(p12), del(12)(q21), inv(13)(p12q21), der(15)t(5;15)(q14;q21), add(15)(p11)add(15)(q2?), der(18)t(14;18)(q32;q21), add(22)(p11), +2mar; carries t(14;18) effecting IGH-BCL2 with non-IG, MYC rearrangement present in markers; published karyotype not available |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |