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| 产品名称： | CHP-134 |
|---|---|
| 商品货号： | TS260042 |
| <strong>Cell line </strong>： | CHP-134 |
| DSMZ no. ： | TS260042 |
| Species ： | human (Homo sapiens) |
| Cell type ： | neuroblastoma |
| Origin ： | established in 1973 from an autopsy specimen of a 18-month-old boy with stage IV neuroblastoma after chemotherapy and irradiation treatment; described in the literature to contain three homogeneously staining regions with amplified copies of N-myc and to be growth-inhibited by methionine aminopeptidase 2 inhibition |
| Reference(s) ： | 14936, 15182, 15184 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | polymorph cells growing adherently in monolayers; image; image; image |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/80 cm2 flask; split confluent culture 1:5 to 1:10 every 3-4 days (trypsin should not be used, cells can be detached by tapping the flask or by pipetting) |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30 hours |
| Harvest ： | cell harvest of ca. 15-20 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |