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| 产品名称： | CRO-AP6 |
|---|---|
| 商品货号： | TS260044 |
| <strong>Cell line </strong>： | CRO-AP6 |
| DSMZ no. ： | TS260044 |
| Species ： | human (Homo sapiens) |
| Cell type ： | B cell lymphoma |
| Origin ： | established at diagnosis from the pleural effusion of a 26-year-old Caucasian HIV + man with primary effusion lymphoma (PEL) in 1999; described in the literature to be HHV-8 +, EBV - and to display biallelic inactivation of the DNA repair enzyme gene O6-methylguanine-DNA methyltransferase (MGMT) by promoter methylation |
| Reference(s) ： | 14419 |
| Biosafety level ： | 2 |
| Risk assessment ： | The cell line carries HHV-8 sequences which were detected by PCR and Southern blot. We did not determine the infection mode of this herpes virus and cannot exclude the secretion of the viruses. The cell line is thus categorized biosafety level 2. |
| Permissions and restrictions ： | A, B |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round to pleomorphic cells growing in small clumps in suspension; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/ml in a 24-well-plate; optimal split ratio of 1:2 to 1:3 every 2-3 days; maintain at 0.5-1.0 x 106 cells/ml; after thawing, viability may drop to 50-60%, but cells recover after 4-5 days; during the first days of culture change medium only |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 48 hours |
| Harvest ： | maximum densitiy of ca. 2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD37 +, CD38 +, cyCD79a -, CD80 -, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda - |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype; 48(45-48)<2n>XY, +16, +mar, der(11)t(4;11)(q28;q24), der(13)dup(13)(q1?q3?)t(13;14)(q3?;q21), der(14)t(13;14)(q3?;q21), add(16)(q24)(hsr), i(21)(q10); carries hsr on der(13) and der(16); matches published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HHV-8 +, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -, SMRV - |