微信小李
微信小章
产品简介
购买须知
| 产品名称： | DND-39 |
|---|---|
| 商品货号： | TS260047 |
| <strong>Cell line </strong>： | DND-39 |
| DSMZ no. ： | TS260047 |
| Species ： | human (Homo sapiens) |
| Cell type ： | Burkitt lymphoma |
| Origin ： | established from the pleural effusion of a 28-year-old man with American-type Burkitt lymphoma in 1977 |
| Reference(s) ： | not published |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round to polygonal cells, growing singly or in small clusters in suspension; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.3-0.5 x 106 cells/ml, optimal split ratio of 1:3 every 2-3 days, maintain at ca. 0.5-1 x 10 6 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-50 hours |
| Harvest ： | maximum densitiy of ca. 2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Plasmocin, then negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda + |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human hypotetraploid karyotype with 4% polyploidy; 86(83-89)<4n>XXYY, -2, -4, -10, -10, -12, -14, -16, -16, +22, +22, der(5)t(2;5)(p24;q14)x2, del(6)(q15)x2 , t(8;14)(q24;q32)x2, add(14)(q32), der(18)add(18)(q21)x2; carries t(8;14) effecting IGH-MYC rearrangement; no reference karyotype available |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |