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| 产品名称： | NALM-16 |
|---|---|
| 商品货号： | TS260102 |
| <strong>Cell line </strong>： | NALM-16 |
| DSMZ no. ： | TS260102 |
| Species ： | human (Homo sapiens) |
| Cell type ： | B cell precursor leukemia |
| Origin ： | established from the peripheral blood of a 12-year-old girl with acute lymphoblastic leukemia (ALL) at relapse in 1977; cells are described in the literature to carry a near haploid karyotype |
| Reference(s) ： | 16561, 16562 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing singly and in small clusters in suspension and partly adherent; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/ml; maintain at ca. 1-1.5 x 106 cells/ml; split saturated culture 1:2-3 every 2-3 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 36 hours |
| Harvest ： | maximum density of ca. 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Plasmocin, then negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD80 -, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype; 52(49-53)<2n>XX, +14, +14, +18, +18, +21, +21, del(X)(q13); clonally related to published near haploid karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |