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| 产品名称： | BC-2 |
|---|---|
| 商品货号： | TS260104 |
| <strong>Cell line </strong>： | BC-2 |
| DSMZ no. ： | TS260104 |
| Species ： | human (Homo sapiens) |
| Cell type ： | B cell lymphoma |
| Origin ： | established in 1992 from the malignant effusion samples of a 31-year-old man at diagnosis of AIDS-related primary effusion lymphoma (PEL); cells are reported to be positive for human herpesvirus type 8 (HHV-8) and Epstein-Barr virus (EBV) while negative for human immunodeficiency virus (HIV) and human T-cell leukemia virus I/II (HTLV-I/II) |
| Reference(s) ： | 14483, 15928, 15929 |
| Biosafety level ： | 2 |
| Risk assessment ： | The cell line carries HHV-8 sequences which were detected by PCR and Southern blot. We did not determine the infection mode of this herpes virus and cannot exclude the secretion of the viruses. The cell line is also positive for EBV (HHV-4) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were also positive by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as lytic associated with production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is thus categorized biosafety level 2. |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round or ovoid cells variable in size growing singly or in clumps; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml in 24-well plates; maintain at ca. 0.4-1.0 x 106 cells/ml; optimal split ratio of 1:2 to 1:3 every 2-5 days; cells grow best in 24-well plates |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-70 hours |
| Harvest ： | cell harvest of ca. 2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, cyCD79a -, CD80 -, CD138 +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | human flat-moded hypertetraploid karyotype with near diploid sideline; 97-99<4n>XXYY,-2, +10, +12, +12, -14, -14, -17, -18, +19, +19, -21, +8-10 mar, add(X)(q24)x2, del(7)(q21.3 )x2, add(11)(q22), add(11)(q24) , add(12)(q13), add(12)(q24)x1-2, der(14)t(7;14)(q21;p11), der(14)t(14;2)(q32;q21)x2-4, add(16)(q21)x1-2; matches published karyotype |
| Viruses ： | PCR: EBV +, HBV -, HCV -, HHV-8 +, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |