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| 产品名称： | NALM-21 |
|---|---|
| 商品货号： | TS260105 |
| <strong>Cell line </strong>： | NALM-21 |
| DSMZ no. ： | TS260105 |
| Species ： | human (Homo sapiens) |
| Cell type ： | B cell precursor leukemia |
| Origin ： | established from a bone marrow specimen of a 63-year-old man with acute undifferentiated leukemia (AUL) at relapse in 1990; cells were described in the literature to carry two Philadelphia chromosomes t(9;22) with a BCR-ABL1 e1-a2 fusion gene; the cell line is a serial sister cell line of NALM-20 (ACC 681) |
| Reference(s) ： | 15848 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing singly and in clusters in suspension; image; image |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.2-2.0 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 2-3 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | maximum densitiy of ca. 3 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 +, CD19 +, CD38 +, CD80 -, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile (identical to that of sister cell line NALM-20) |
| Cytogenetics ： | human near-diploid karyotype; 46(44-48)<2n>X, -Y, +8, inv(4)(p14q35), i(8)(q10)x2, t(9;22)(q34;q11); carries t(9;22) effecting BCR-ABL1 rearrangement; resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |