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| 产品名称： | NALM-20 |
|---|---|
| 商品货号： | TS260106 |
| <strong>Cell line </strong>： | NALM-20 |
| DSMZ no. ： | TS260106 |
| Species ： | human (Homo sapiens) |
| Cell type ： | B cell precursor leukemia |
| Origin ： | established in 1989 from the peripheral blood of a 62-year-old man at diagnosis of acute undifferentiated leukemia (AUL); ; cells were described in the literature to carry a Philadelphia chromosome t(9;22) with a BCR-ABL1 e1-a2 fusion gene; the cell line is a serial sister cell line of NALM-21 (ACC 682) |
| Reference(s) ： | 15848 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | small round cells growing singly or occasionally in clumps; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 0.8-1.5 x 106 cells/ml in 24-well plates; maintain at ca. 0.6-1.5 x 106 cells/ml; optimal split ratio of 1:2 to 1:3 every 2-5 days; cells grow slowly after recovery from liquid nitrogen |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 60-80 hours |
| Harvest ： | cell harvest of ca. 2.5-3.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 +, CD19 +, CD38 +, cyCD79a -, CD80 -, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile (identical to that of sister cell line NALM-21) |
| Cytogenetics ： | human hyperdiploid karyotype; 48(45-48)<2n>XY, +8, +8, inv(4)(p14q35), del(8)(p12)x2, t(9;22)(q34;q11); carries t(9;22) effecting BCR-ABL1 rearrangement; resembles published karyotype |
| Molec. Genetics ： | expression of fusion gene BCR-ABL1 (e1-a2) confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |