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| 产品名称： | OCI-LY7 |
|---|---|
| 商品货号： | TS260108 |
| <strong>Cell line </strong>： | OCI-LY7 |
| DSMZ no. ： | TS260108 |
| Species ： | human (Homo sapiens) |
| Cell type ： | B cell lymphoma |
| Origin ： | established from the peripheral blood sample of a 48-year-old man with B-cell non-Hodgkin lymphoma (B-NHL, diffuse large B-cell lymphoma, DLBCL, stage 2A, at relapse) in 1984; cells were described in the literature to be EBV-negative, to carry the t(8;14) translocation effecting MYC-IGH and a TP53 point mutation. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14427, 15026, 15976, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round to polymorph cells growing in clusters; image; image |
| Medium ： | 80-90% Iscoves MDM + 10-20% h.i. FBS |
| Subculture ： | seed out at ca. 0.3-0.5 x 106 cells/ml in flasks; maintain at ca. 0.5-3.0 x 106 cells/ml; split culture 1:3 every 2 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 20-24 hours |
| Harvest ： | maximum density at ca. 3.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 -, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | human near-diploid karyotype with 3% polyploidy; 46(42-48)<2n>XY, inv(6)(q12q21), t(8;14)(q24;q32), del(18)(q21.1); carries t(8;14) effecting MYC-IGH rearrangement; matches published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |