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| 产品名称： | BL-100 |
|---|---|
| 商品货号： | TS260112 |
| <strong>Cell line </strong>： | BL-100 |
| DSMZ no. ： | TS260112 |
| Species ： | human (Homo sapiens) |
| Cell type ： | Burkitt lymphoma |
| Origin ： | established from the bone marrow of a Caucasian woman with EBV-negative Burkitt lymphoma with translocation t(8;22) after EBV infection in culture |
| Reference(s) ： | not published |
| Biosafety level ： | 2 |
| Risk assessment ： | The cell line is positive for EBV (HHV-4) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were also positive by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as lytic associated with a production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is thus categorized biosafety level 2. |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | mostly single round cells in suspension, partly in small clusters; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.3-2.0 x 106 cells/ml; split saturated culture 1:3 every 3-4 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | maximum densitiy of ca. 2.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Baytril (enrofloxacin), then negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype; 47(44-48)<2n>XX, +12, del(1)(q23), der(3)t(1;3)(q23;q2?2), t(8;22)(q24;q11); carries t(8:22) effecting rearrangement of IGL with MYC; matches unpublished karyotypic data |
| Viruses ： | PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -, SMRV + |