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| 产品名称： | HD-MAR-2 |
|---|---|
| 商品货号： | TS260116 |
| <strong>Cell line </strong>： | HD-MAR-2 |
| DSMZ no. ： | TS260116 |
| Species ： | human (Homo sapiens) |
| Cell type ： | T cell leukemia |
| Origin ： | established in 1977 from the pleural effusion of a 20-year-old man with a recurrent T-lymphoblasic non-Hodgkin lymphoma after treatment of the originally diagnosed Hodgkin lymphoma |
| Reference(s) ： | 15845 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing singly and in clusters in suspension; image; image; image |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml, maintain at ca. 0.2-2 x 106 cells/ml, split saturated culture 1:2 to 1:3 every 2-3 days; upon thawing viability decrease to about 60%, but cells recover within a week; it is recommended to culture the cells with 20% FBS during the first week |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | maximum density at ca. 2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD2 +, CD3 +, CD4 +, CD5 +, CD6 +, CD7 +, CD8 +, CD13 -, CD19 -, CD34 -, TCRalpha/beta +, TCRgamma/delta -; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | human flat-moded hypertetraploid karyotype; 92-99<4n>XXYY, -4, +8, +19, +20, +20, +21, +21, +22, +22, del(1)(q21), add(6)(q11)/add(6)(q25), der(9)t(9;10)(p22;?p13)t(9;14)(q35;q11.2)x2, der(14)t(9;14)x2-4, der(17;19)(q10;q10)x2, add(19)(p11), del(19)(q11), del(20)(q11); carries t(9;14) directing upregulation of truncated NOTCH1 by TRA/TRD (TCR@A/D) juxtaposition; resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |