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| 产品名称： | UCSD-AML1 |
|---|---|
| 商品货号： | TS260119 |
| <strong>Cell line </strong>： | UCSD-AML1 |
| DSMZ no. ： | TS260119 |
| Species ： | human (Homo sapiens) |
| Cell type ： | acute myeloid leukemia |
| Origin ： | established from the bone marrow of a 73-year-old woman with acute myeloid leukemia at relapse in 1989; cells were described in the literature to be cytokine-dependent (GM-CSF, IL-6, or M-CSF) and to carry the translocations t(3;3)(q21;q26) and t(12;22)(p13;q12) leading to MECOM (EVI1) overexpression and to ETV6-MN1 (TEL-MN1) fusion gene, respectively |
| Reference(s) ： | 16052, 16053 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round to polygonal single cells with variable sizes in suspension, a few cells are lightly adherent; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS + 10 ng/ml GM-CSF |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.2-1.0 x 106 cells/ml; split culture 1:3 to 1:5 every 3-4 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-60 hours |
| Harvest ： | maximum density at ca. 1 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD4 +, CD13 +, CD14 -, CD15 -, CD19 -, CD33 +, CD34 +, cyCD68 +, HLA-DR +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | human flat-moded hypodiploid karyotype with 10% polyploidy; 45(42-45)<2n>XX,-7, t(3;3)(q21;q26), t(12;22)(p13;q12); carries t(3;3) and t(12;22) effecting MN1 and ETV6/MN1 rearrangements, respectively; precisely matches published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |