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| 产品名称： | YNH-1 |
|---|---|
| 商品货号： | TS260120 |
| <strong>Cell line </strong>： | YNH-1 |
| DSMZ no. ： | TS260120 |
| Species ： | human (Homo sapiens) |
| Cell type ： | acute myeloid leukemia |
| Origin ： | established from the peripheral blood of a 46-year-old man with acute myeloid leukemia (AML M1) at diagnosis in 1994; cells were described in the literature to be constitutively cytokine-dependent and cytokine-responsive and to carry the translocation t(16;21)(p11;q22) leading to FUS-ERG (TLS-ERG) fusion gene |
| Reference(s) ： | 16260, 16261 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single round cells growing in suspension, some cells are loosely adherent; image; image ; image |
| Medium ： | 60% RPMI 1640 + 20% h.i. FBS + 20% vol conditioned medium of cell line 5637 (DSM ACC 35) or 10 ng/ml G-CSF |
| Subculture ： | seed out at ca. 0.5-1 x 106 cells/ml in 24-well plates; maintain at ca. 0.5-1 x 106 cells/ml; split saturated culture 1:2 every 3-4 days. CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus, the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines. |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 80 hours |
| Harvest ： | maximum density at ca. 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD4 +, CD13 +, CD14 -, CD15 -, CD19 -, CD20 -, CD33 +, CD34 +, cyCD68 +, HLA-DR -;image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 4% polyploidy; -47(44-48)<2n>XY, +19, add(7)(q35), der(16)t(1;16)(q12;q12)t(16;21)(p11;q22), der(21)t(16;21)(p11;q22; carries complex t(16;21) effecting rearrangement of FUS (TLS) with ERG; matches published karyotype |
| Molec. Genetics ： | expression of fusion gene FUS-ERG (TLS-ERG) confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |