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| 产品名称： | SKNO-1 |
|---|---|
| 商品货号： | TS260124 |
| <strong>Cell line </strong>： | SKNO-1 |
| DSMZ no. ： | TS260124 |
| Species ： | human (Homo sapiens) |
| Cell type ： | acute myeloid leukemia |
| Origin ： | established in 1990 from the bone marrow of a 22-year-old man with acute myeloid leukemia (AML M2) in second relapse. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 16563, 14357, 16564, 14482, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round to polymorphic cells growing singly or occasionally in small clusters in suspension; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS + 10 ng/ml GM-CSF |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.2-2.0 x 106 cells/ml; split culture 1:2 to 1:3 every 2-3 days; alternatively to GM-CSF, 5-10% conditioned medium of the cell line 5637 may be used. CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus, the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines. |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 35-50 hours |
| Harvest ： | maximum density at ca. 2.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD4 +, CD13 +, CD14 -, CD15 -, CD19 -, CD33 +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | clonally heterogeneous human near-diploid karyotype; 46(42-48)<2n>X, -X, +5, +6, +16, add(2)(q22), i(5)(p10), add(6)(q27) or i(6)(p10), der(7)t(7;9)(q35.2;q21), t(8;21)(q22;q22), ider(9)(p10)add(9)(p21), add(11)(p15) or inv(11)(p15q24), der(16;17)(q10;q10), der(17;19)(p10;q10); resembles published karyotype; carries t(8;21) effecting RUNX1-RUNX1T1 (AML1-ETO) rearrangement |
| Molec. Genetics ： | expression of fusion gene RUNX1-RUNX1T1 (AML1-ETO) confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |