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| 产品名称： | ESO-51 |
|---|---|
| 商品货号： | TS260137 |
| <strong>Cell line </strong>： | ESO-51 |
| DSMZ no. ： | TS260137 |
| Species ： | human (Homo sapiens) |
| Cell type ： | esophageal adenocarcinoma |
| Origin ： | established from the primary tumor of a 74-year-old white man with esophageal adenocarcinoma in 2000 |
| Reference(s) ： | 16055 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | polymorphic cells growing in clusters in suspension |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | seed out at ca. 0.1-0.2 x 106 cells/ml; split culture 1:2 to 1:3 every 2-3 days; cell clusters are difficult to disperse and cell counting may be difficult; viability may drop below 60% after recovery from liquid nitrogen, but cells recover after 4-5 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | human flat bimodal hyperdiploid/hypertetraploid karyotype; 51-63/90-121<4n>XXXX, -Y, -Y, -Y, -Y, + 5-12mar; add(X)(p11)x2, del(2)(q21)x2, dup(2)(q11q24)i(2)(q10), dup(3)(q24q29), i(3)(p10), add(3)(p25), add(7)(q21)(hsr)x2, del(13)(q11q13), add(17)(q 25), add(21)(p11) |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/-II -, MLV - |