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| 产品名称： | MKPL-1 |
|---|---|
| 商品货号： | TS260139 |
| <strong>Cell line </strong>： | MKPL-1 |
| DSMZ no. ： | TS260139 |
| Species ： | human (Homo sapiens) |
| Cell type ： | acute megakaryoblasic leukemia |
| Origin ： | established in 1989 from the bone marrow of a 66-year-old man with acute megakaryoblastic leukemia (AML M7). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 16259, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round to polymorphic cells growing singly and in clumps in suspension, 1% giant cells; image; image |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.2-0.8 x 106 cells/ml; split saturated culture 1:2 to 1:4 every 2-3 days; culture may contain a high amount of cell debris |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-60 hours |
| Harvest ： | maximum density at ca. 1 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD13 +, CD14 -, CD15 -, CD19 -, CD33 +, CD34 +, cyCD68 +, HLA-DR -; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | human flat-moded near tetraploid karyotype with 5% polyploidy; 86-93<4n>XX, -Y, -Y, +1, -4, +5, +6, -9, +13, -16, -18, -18, +19, +21, -22, +1-3mar; del(1)(q11), i(5)(p10)x2, del(6)(q21q27), add(7)(p11), dup(13)(q21q31), i(del)(13)(q11q31)x2, i(18)(q10), add(19)(p13), del(19)(q11)x1-2, add(21)(q21.2)x1-2, qdp(21)(q22.?1q22.?3)x2; resembles published karyotype; qdp(21) targets rearrangement and amplification of RUNX1 (AML1) |
| Molec. Genetics ： | expression of fusion gene RBM6-CSF1R confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |