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| 产品名称： | OE-19 |
|---|---|
| 商品货号： | TS260147 |
| <strong>Cell line </strong>： | OE-19 |
| DSMZ no. ： | TS260147 |
| Species ： | human (Homo sapiens) |
| Cell type ： | esophageal adenocarcinoma |
| Origin ： | established from the primary tumor of a 72-year-old Caucasian man with esophageal adenocarcinoma in 1993; cell line also known as JROECL19 |
| Reference(s) ： | 16110, 16055 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial cells growing adherently as monolayer; image; image |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | seed out at ca. 2 x 106 cells/80 cm2 flask; split confluent culture 1:5 every 2-3 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-60 hours |
| Harvest ： | cell harvest of ca. 10 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | human flat-moded hypotriploid karyotype with 4% polyploidy; 58-62<3n>XX, -Y, +1, -4, -5, +8, -10, -14, -14, -15, -15, -17, -18, -18, -21, -21, -22, -22, +6mar; del(X)(p11)ins(X;?)(q11?), add(1)(p11), del(2)(q12), add(3)(p21), add(4)(q11), del(p14), der(4)t(4;4)(p11;q24), del(5)(q11q21), der(6)add(6)(p11)add(6)(q25), add(6)(q11), i(6)(p10), del(7)(q12), add(9(p21), add(10)(p11), del(10)(p12), i(11)(p10), add(12)(p11), i(12)(p10), der(12;13)(p10;q10), add(13)(q32), inv(15)(p11q21), der(16)t(1;16)(q24;p13), add(18)(q23) |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/-II -, MLV - |