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| 产品名称： | IPEC-1 |
|---|---|
| 商品货号： | TS260153 |
| <strong>Cell line </strong>： | IPEC-1 |
| DSMZ no. ： | TS260153 |
| Species ： | originally confirmed as pig by species PCR and recently as sus scrofa origin by COI DNA barcoding |
| Cell type ： | intestine (jejunum/ileum) |
| Origin ： | established from normal intestinal epithelium cells isolated from both jejunum and ileum of a neonatal pig |
| Reference(s) ： | 16042, 16044, 16043 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid cells growing adherently in monolayers and clusters; image; image |
| Medium ： | 90% mixture of Hams F12 + Dulbeccos MEM (at 1:1) + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2 flask; split almost confluent culture 1:4 once or twice a week using trypsin/EDTA for about 10 minutes |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30 hours |
| Harvest ： | cell harvest of ca. 4-5 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |