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| 产品名称： | OE-33 |
|---|---|
| 商品货号： | TS260157 |
| <strong>Cell line </strong>： | OE-33 |
| DSMZ no. ： | TS260157 |
| Species ： | human (Homo sapiens) |
| Cell type ： | esophageal adenocarcinoma |
| Origin ： | established from the primary tumor of a 73-year-old white woman with Barrett-esophageal adenocarcinoma in 1993; cells are described in the literature to produce tumors in nude mice; cell line also known as JROECL33 |
| Reference(s) ： | 16055, 16110 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid cells growing adherently in single cells and clusters; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 2 x 106 cells/175 cm2; split confluent culture 1:10 every 4-5 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30 hours |
| Harvest ： | cell harvest of ca. 20 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | human flat-moded hypertetraploid karyotype; 92-100<4n>XX, -X, -X, +1, -4, +5, +6, +7, +7, +7, +7, +8, +10, +10, -13, -13, -13, +16, +16, +16, -17, -18, -18, -19, +20, +20, +20, +20, -21, -22, -22, +6mar; add(1)(p13)x2, i(3)(q10)x1-2, del(5)(p14.2), add(6)(q22), add(7)(q34)x3, der(11)add(11)(q23)i(11)(q10), add(14)(p11) |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/-II -, MLV - |