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| 产品名称： | HH |
|---|---|
| 商品货号： | TS260161 |
| <strong>Cell line </strong>： | HH |
| DSMZ no. ： | TS260161 |
| Species ： | human (Homo sapiens) |
| Cell type ： | cutaneous T cell lymphoma |
| Origin ： | established in 1986 from peripheral blood mononuclear leucocytes of a 61-year-old man with an aggressive, fatal cutaneous T-cell lymphoma following a long history of psoriasis developing into nodular ulcerating skin lesions 7 months prior to diagnosis of lymphoma. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 17683, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round or irregularly shaped cells extruding long processes growing singly or in clumps in suspension together with large solitary ovoid cells; image; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml in 24-well plates; split 1:2 to 1:4 every 2-4 days; maintain at 0.3-0.7 x 106 cells/ml; cells can be transfered to 80 cm2 flasks when the cells recovered from storage in liquid nitrogen |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-70 hours |
| Harvest ： | maximum density at ca. 1.2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD2 +, CD3 +, CD4 +, CD5 +, CD6 +, CD7 -, CD8 -, CD13 -, CD19 -, CD30 +, CD34 -, HLA-DR +, TCRalpha/beta +, TCRgamma/delta -; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | human flat-moded near-tetraploid karyotype with near-diploid sideline and 3% polyploidy; 86-104<4n>XXX, -Y, +13, +13, -15, -15, +18, +18, -21, -21, der(1)del(1)(p33)del(1)(q31)x2, der(3)t(2;3)(q25;p25)x2-3, t(3;12)(q11;q25)x2, der(4)t(1;4)(q25;q34)x2, t(6;14)(q25;q24)x2, der(9)t(9;10)(q34;q23)x2-3, del(10)(q25)x1-2, der(10)t(1;10)(q25;q23)x2, del(13)(q11q31.2)x2, der(13)t(13;21)(q33;q22)x2; no karyotype published hitherto |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |