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| 产品名称： | PACADD-135 |
|---|---|
| 商品货号： | TS260164 |
| <strong>Cell line </strong>： | PACADD-135 |
| DSMZ no. ： | TS260164 |
| Species ： | human (Homo sapiens) |
| Cell type ： | pancreas ductal mucinous adenocarcinoma |
| Origin ： | established from a lymph node metastasis of a 48-year-old Caucasian woman with irresectable pancreatic ductal mucinous adenocarcinoma; cells were described in the literature to carry a G12V KRAS mutation |
| Reference(s) ： | 16465 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid cells growing adherently in monolayers |
| Medium ： | 90% mixture of DMEM and Keratinocyte SFM (at 1:1) + 10% h.i. FBS or 90% DMEM + 10% FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2 flask; split confluent culture 1:3 to 1:4 once a week using trypsin/EDTA for 5 minutes |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 72 hours |
| Harvest ： | cell harvest of ca. 13 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |