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| 产品名称： | ESO-26 |
|---|---|
| 商品货号： | TS260165 |
| <strong>Cell line </strong>： | ESO-26 |
| DSMZ no. ： | TS260165 |
| Species ： | human (Homo sapiens) |
| Cell type ： | esophageal adenocarcinoma |
| Origin ： | established from the primary tumor (pT4N1M1) of a 56-year-old Caucasian man with esophageal adenocarcinoma in 2000; cells are described in the literature to carry a p53 mutation (C742T) |
| Reference(s) ： | 16055 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | ovoid cells growing semiadherently, singly or in clumps |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | seed out at ca. 2-5 x 106 cells/175 cm2 flask; split culture 1:2 to 1-6 every 2-6 days; cells can be detached by tapping the flask or by vigorous shaking |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 35-50 hours |
| Harvest ： | cell harvest of ca. 100 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | human hypodiploid karyotype; 39(36-40)<2n>X, -Y, +2, -3, -5, -11, -12, -15, -16, -19, -20, -22, +mar add(X)(p22), del(1)(p32), add(12)(p13), der(13;21)(q10;q10), i(13)(q10), add(17)(p12), add(?18)(q21), add(21)(p11) |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/-II -, MLV - |