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| 产品名称： | KASUMI-3 |
|---|---|
| 商品货号： | TS260168 |
| <strong>Cell line </strong>： | KASUMI-3 |
| DSMZ no. ： | TS260168 |
| Species ： | human (Homo sapiens) |
| Cell type ： | acute myeloid leukemia |
| Origin ： | established from blast cells of the bone marrow of a 57-year-old Japanese man with minimally differentiated acute myeloblasic leukemia (AML M0); cells were described in the literature to carry a t(3;7) leading to alteration of MECOM-TRB (EVI1-TRB) genes and to MECOM (EVI1) overexpression |
| Reference(s) ： | 16469 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round to polymorphic cells growing singly and in small clumps in suspension; image; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | start with ca. 0.5-1 x 106 cells/ml in 24-well plates; later, maintain at ca. 0.3-1 x 106 cells/ml in flasks; split saturated culture 1:2 every 3-4 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 3-4 days |
| Harvest ： | maximum density at ca. 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD2 -, cy/smCD3 -, CD4 +, CD5 -, CD7 +, CD8 -, CD13 +, CD14 -, CD15 -, CD19 -, CD20 -, CD33 +, CD34 +, cyCD68 +, HLA-DR +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | human pseudodiploid karyotype with 9% polyploidy; 46(43-47)<2n>XY, der(2)t(2;12)(p15;p13), der(3)t(3;7)(q26;q35)t(7;8)(q22;q23), der(4)t(4;8)(q13;q21), del(5)(q15), der(5)t(5;10)(q33;q24), der(7)t(3;7)(q26;q35)del(7)(p1?), der(8)t(4;8)(q13;q12), del(9)(q32), del(10)(q24), add(12)(p11), add(16)(q13.2) ; carries rearrangement of MECOM (EVI1) with TRB (TCRB); resembles published karyotypes |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |