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| 产品名称： | LAZ-221 |
|---|---|
| 商品货号： | TS260172 |
| <strong>Cell line </strong>： | LAZ-221 |
| DSMZ no. ： | TS260172 |
| Species ： | human (Homo sapiens) |
| Cell type ： | B cell precursor leukemia |
| Origin ： | established in 1977 from the peripheral blood of a 24-year-old Caucasian woman with "null cell" acute lymphoblastic leukemia (null-ALL) at diagnosis |
| Reference(s) ： | 16452 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | small round cells, growing singly or in small clumps; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 1.5 x 106 cells/ml in 24-well plates; cells grow slowly during the first week, do not split cell culture and only exchange spent medium partially during the first week; maintain at ca. 1-2 x 106 cells/ml; split culture 1:2 every 2-3 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-80 hours |
| Harvest ： | maximum density at ca. 3 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD4 -, CD5 +, CD7 -, CD8 -, CD10 +, CD13 -, CD19 +, CD20 -, CD34 -, CD37 -, CD38 +, CD80 -, CD138 +, HLA-DR +, TCRalpha/beta -, TCRgamma/delta -, sm/cyIgG -, sm/cyIgM +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | human hypodiploid karyotype; 45(41-46)<2n>XX, -12, der(2)t(2;18)(q36;q21), der(9)t(9;12)(p12;q11), del(18)(q22); resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |