微信小李
微信小章
产品简介
购买须知
| 产品名称： | GRANTA-452 |
|---|---|
| 商品货号： | TS260175 |
| <strong>Cell line </strong>： | GRANTA-452 |
| DSMZ no. ： | TS260175 |
| Species ： | human (Homo sapiens) |
| Cell type ： | B cell leukemia |
| Origin ： | derived from a case of B cell acute lymphoblastic leukemia (B-ALL) following transformation from follicular B cell non-Hodgkin lymphoma (B-NHL); described in the literature to carry a t(2;9) (p1;p13) and a t(9;14;18) (p13;q32;q21), to exhibit a biallelic deletion of CDKN2A as well as a BCL2 MBR rearrangement |
| Reference(s) ： | 16493, 15064 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing singly in suspension; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/ml; maintain at ca. 0.5-1 x 106 cells/ml; split culture 1:2 to 1:4 every 2-3 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 48 hours |
| Harvest ： | maximum density at ca. 2-3 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD20 -, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, HLA-DR +, sm/cyIgG +, sm/cyIgM -, sm/cykappa -, sm/cylambda +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 5% polyploidy; 49(46-50)<2n>XXY, +X, +7, +11, der(1)t(1;7)(p36;p15), der(2)t(2;4)(p21;p15.2), der(3)t(1;3)(p26;q11), der(4)t(4;6)(p11;q22), del(6)(q13), trp(8)(q22q24), t(8;22)(q24;q11), der(9)t(2;9)(p13;p15.2), der(9)t(9;14)(p13;q22)t(14;18)(q32;q21), der(14)t(9;14)(p21;q22), t(16;19)(p13;p13), der(18)t(14;18)(q32;q21), der(20)t(9;20)(p21;p13)ins(9;?)(p21.2;?); carries IGH/BCL2 and IGL/MYC rearrangements, together with a complex series of rearrangements effecting deletion of CDKN2 (homozygous) and PAX5 (hemizygous); consistent with published karyotype data |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |