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| 产品名称： | MOLM-1 |
|---|---|
| 商品货号： | TS260178 |
| <strong>Cell line </strong>： | MOLM-1 |
| DSMZ no. ： | TS260178 |
| Species ： | human (Homo sapiens) |
| Cell type ： | chronic myeloid leukemia |
| Origin ： | established from the bone marrow of a 41-year-old man with chronic myelogenous leukemia (CML) in blast crisis; cells are described in the literature to display megakaryocytic features and to be inducible to megakaryocytic differentiation; cells carry the Philadelphia chromosome with an e13-a2 (b2-a2) BCR-ABL1 fusion gene and an inv(3) leading to MECOM (EVI1) overexpression |
| Reference(s) ： | 16545, 16546, 14484 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing singly in suspension; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml in 24-well plate; cells may grow slowly in the first week after thawing; split saturated culture 1:2 every 2-3 days; maintain at ca. 0.5-1.5 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 60-70 hours |
| Harvest ： | maximum density at ca. 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD4 +, CD13 +, CD14 -, CD15 -, CD19 -, CD33 +, CD34 +, HLA-DR +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | human flat-moded near-triploid karyotype; 66-70<3n>X, -X/Y, -X/Y, +1, -7, +13, -16, -18, +22, del(5)(q11q34.2), inv(3)(q21q26)x2, der(9)t(9;22)(q34;q11), der(22)t(9;22)(q34;q11)x2; carries BCR-ABL1 and MECOM (EVI1) rearrangements; matches published karyotype |
| Molec. Genetics ： | expression of fusion gene BCR-ABL1 e13-a2 (b2-a2) confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |