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| 产品名称： | NCEB-1 |
|---|---|
| 商品货号： | TS260182 |
| <strong>Cell line </strong>： | NCEB-1 |
| DSMZ no. ： | TS260182 |
| Species ： | human (Homo sapiens) |
| Cell type ： | B cell lymphoma |
| Origin ： | established in 1987 by Epstein-Barr virus (EBV) transformation of neoplastic peripheral blood cells from a 57-year-old man with centroblastic-centrocytic diffuse B-cell non-Hodgkin lymphoma (B-NHL; mantle cell lymphoma); cells stably carry 5-8 mouse chromosomes and a t(11;14) leading to alteration of CCND1 (BCL1) and IGH genes |
| Reference(s) ： | 16466, 16467, 16468, 15229 |
| Biosafety level ： | 2 |
| Risk assessment ： | The cell line is positive for EBV (HHV-4) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 was also positive by western blot in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as lytic associated with a production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is thus categorized biosafety level 2. |
| Permissions and restrictions ： | A, B |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round to polymorphic cells growing singly or in clumps in suspension, some cells are adherent; image |
| Medium ： | 85% RPMI 1640 + 15% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; split culture 1:2 every 2-3 days; maintain at ca. 0.5-1.3 x 106 cells/ml; adherent cells can be detached by tapping the flask |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-70 hours |
| Harvest ： | maximum density at ca. 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD5 +, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 +, HLA-DR +, cyIgM +, cyIgG -, cykappa -, cylambda +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | unique predominantly human, human/mouse hybrid flat-moded hypotetraploid karyotype; 82-91<4n>XX, -Y, -Y, -8, -12, -13, -13, -15, -15, -17, +6 - 7 mouse chr., del(X)(q21), der(5)t(5;12)(p15;q13)x2, del(7)(p21)x2, der(9)t(X;9)(q25;q32)t(X;13)(q27;q21)x2, add(10)(q25)x1-2, der(11)t(11;14)(q13;q32), t(11;14)(q13;q32)x2; resembles published karyotype; carries t(11;14) effecting rearrangement of IGH with CCND1; mouse chromosomes may have been acquired during early passage on mouse macrophage feeder layers |
| Viruses ： | PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV + |