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| 产品名称： | OCI-LY1 |
|---|---|
| 商品货号： | TS260183 |
| <strong>Cell line </strong>： | OCI-LY1 |
| DSMZ no. ： | TS260183 |
| Species ： | human (Homo sapiens) |
| Cell type ： | B cell lymphoma |
| Origin ： | established from the bone marrow of a 44-year-old man with B-cell non-Hodgkin lymphoma (B-NHL; diffuse large cell), stage 4B at relapse in 1983; assigned to GCB-like lymphoma subtype (germinal center B-cell) |
| Reference(s) ： | 14427, 15976, 16454, 16455, 16456, 17354 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | small round to polymorph cells growing singly in suspension; image; image; image |
| Medium ： | 80% Iscoves MDM + 20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.5-2 x 106 cells/ml; split saturated culture 1:2 every 2-3 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | maximum density at ca. 2.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD138 -, HLA-DR -, cyIgG -, cyIgM +, cykappa +, cylambda -; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | human flat-moded hyperdiploid karyotype with 6% polyploidy; 47(41-48)<2n>XY, +7, der(4)t(4;7)(p14;q22), der(7)t(4;7)(p14;p14)del(7)(q22), der(8)t(8;13)(p13;q13), der(8)t(8;8)(p22;q11), der(10)t(10;14)(p14;q32)t(14;11)(q32;q24), der(11)t(11;14)(q24;q32)t(14;18)(q32;q21), del(13)(q31), t(14;18)(q32;q21), der(18)t(3;18)(q24;q21)t(18;18)(p12;q21); carries t(14;18) rearrangements, some cryptic, effecting juxtaposition of IGH with BCL2; resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |