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| 产品名称： | MEGAL |
|---|---|
| 商品货号： | TS260186 |
| <strong>Cell line </strong>： | MEGAL |
| DSMZ no. ： | TS260186 |
| Species ： | human (Homo sapiens) |
| Cell type ： | acute megakaryoblastic leukemia |
| Origin ： | derived from a case of acute megakaryocytic leukemia (AML M7); cell line expresses the SET-NUP214 fusion transcript. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 16550, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | small round cells, growing singly in suspension; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/ml in 24-well plate; cells may grow slowly in the first week after thawing; split culture 1:2 twice a week; cells are difficult to culture |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 70 hours |
| Harvest ： | maximum density at ca. 2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD13 -, CD14 -, CD19 -, CD33 +, CD34 +, CD41 -, CD71 +, CD235a -; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype; 49(45-52)<2n>XX, -8, +13, +13, -15, -16, -17, +5 mar, del(2)(q11q22), t(2;15)(p16;q14), der(6)del(6)(p23)t(6;17)(q27;q11), del(9)(q34.11q34.13), ins(13;16)(q31;q13q23), ?r(13)(q31q32)x3, der(14)t(13;14)(p12;q12)t(14;9)(q21;q11), del(16)(q13q23), der(19)t(15;19)(q?;p13); karyotype hitherto unreported; carries cryptic 9q34 deletion effecting SET-NUP214 fusion; rearrangements at 13q31 associated with activation of miR-17~92 cluster |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |