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| 产品名称： | OCI-AML1 |
|---|---|
| 商品货号： | TS260188 |
| <strong>Cell line </strong>： | OCI-AML1 |
| DSMZ no. ： | TS260188 |
| Species ： | human (Homo sapiens) |
| Cell type ： | acute myeloid leukemia |
| Origin ： | established in 1987 from the peripheral blood of a 73-year-old woman with acute myelomonocytic leukemia (AML M4) |
| Reference(s) ： | 15050, 15051, 16751, 16805, 14987 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing singly in suspension; image |
| Medium ： | 60% alpha-MEM (with ribo- and deoxyribonucleosides) + 20% h.i. FBS + 20% vol conditioned medium of cell line 5637 (DSM ACC 35) (or 10-20 ng/ml GM-CSF) |
| Subculture ： | seed out at ca. 0.8-1.0 x 106 cells/ml; maintain at ca. 0.5-1.0 x 106 cells/ml; split saturated culture 1:2 every 3-4 days; possibly no cell growth during first week after thawing; we recommend to culture the cells in 24-well plates; CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines. |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 3-4 days |
| Harvest ： | maximum density at ca. 2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD4 +, CD13 +, CD14 -, CD15 +, CD19 -, CD33 -, CD34 +, cyCD68 +, HLA-DR +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | human diploid karyotype; 46(41-46)<2n>XX; no consistent abnormality detected; lacks t(6;8) present in sister cell line OCI-AML1a |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |