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| 产品名称： | P30-OHKUBO |
|---|---|
| 商品货号： | TS260189 |
| <strong>Cell line </strong>： | P30-OHKUBO |
| DSMZ no. ： | TS260189 |
| Species ： | human (Homo sapiens) |
| Cell type ： | B cell precursor acute lymphoblastic leukemia |
| Origin ： | established from the bone marrow of an 11-year-old girl with acute lymphoblastic leukemia (ALL FAB L2) after 3rd relapse following bone marrow transplantation in 1980 |
| Reference(s) ： | 16668, 14744 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single cells growing in suspension; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.1-2.0 x 106 cells/ml; split saturated culture 1:2 to 1:4 every 2-3 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30 hours |
| Harvest ： | maximum density at ca. 3 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 -, CD38 +, cyCD79a +, CD80 -, CD138 +, HLA-DR +, cyIgG -, cyIgM +, cykappa -, cylambda +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | human flat-moded hypodiploid karyotype; 45(42-46)<2n>X, -X, der(2)t(2;4)(p22;q31), der(3)t(3;21)(q28;q22.2), der(4)t(4;9)(q24;q32), der(6)t(6;14)(q25;q24), der(8)t(4;8)(q24;q24)t(4;13)(q31;q31), der(9)del(9)(p21p23)inv(9)(p21q13)t(2;9)(p22;q13), der(9)t(8;9)(q23;p21), del(12)(q23q24), der(12)t(6;12)(q25;p13), del(14)(q31); related to published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |