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| 产品名称： | KHYG-1 |
|---|---|
| 商品货号： | TS260194 |
| <strong>Cell line </strong>： | KHYG-1 |
| DSMZ no. ： | TS260194 |
| Species ： | human (Homo sapiens) |
| Cell type ： | natural killer cell leukemia |
| Origin ： | established 1997 from the peripheral blood of a 45-year-old woman with aggressive natural killer cell (NK) leukemia; cells were described in the literature to display strong cytotoxic activity and to carry a point mutation in exon 7 of the TP53 (p53) gene. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 16669, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round to polymorph cells growing singly and in clumps in suspension; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS+ 10 ng/ml IL-2 |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.1-0.8 x 106 cells/ml; split saturated culture 1:2 to 1:4 every 2-3 days; viability may be low after thawing, but cells recover during the following 2-3 days, culture should be started with 20% FBS in 24-well-plates; CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines. |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-40 hours |
| Harvest ： | maximum density at ca. 1 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD2 +, cyCD3 +, smCD3 -, CD4 -, CD5 -, CD6 +, CD7 +, CD8 +, CD13 -, CD19 -, CD34 -, TCRalpha/beta -, TCRgamma/delta -; image |
| Fingerprint ： | fluorescent nonaplex PCR revealed a unique STR DNA profile |
| Cytogenetics ： | human highly rearranged flat-moded hypertetraploid karyotype with 2.5% polyploidy; 84-96<4n>XXXX, +8, +8, +8, -9, t(5;6)(q21;q24)x2, der(7)t(4;7)(q31;q33)x2, der(7)dup(7)(q?31q?35)t(5;7)(p15;q35)x2, der(8)t(8;8)(p22;q24)inv(8)(q12q24)x2, der(9;10)(q10;q10), dic(10;20)(p12;q13), der(10)t(10;13)(p11;q11)t(13;16)(q21;q12)x1-2, der(11)t(7;11)(q22;q24)x2, der(15)t(15;16)(p13;q12)x2, der(16)t(13;16)(q21;q12)x2, i(18)(q10)x2, der(20)t(7;20)(q22;q13)x2; copy number increases affecting BCL2/18q21 and MYC/8q24 regions; resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |