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| 产品名称： | CARNAVAL |
|---|---|
| 商品货号： | TS260195 |
| <strong>Cell line </strong>： | CARNAVAL |
| DSMZ no. ： | TS260195 |
| Species ： | human (Homo sapiens) |
| Cell type ： | diffuse large B cell lymphoma |
| Origin ： | established 2010 from the pleural effusion of a patient with diffuse large B cell lymphoma (DLBCL) |
| Reference(s) ： | not published |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single cells growing in suspension; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 2-3 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40-50 hours |
| Harvest ： | maximum density at ca. 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 -, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | human near diploid karyotype; 46(44-47)<2n>XX, +X, -Y, -4, +7, del(X)(p21)/idic r(X)(?p11?q21), der(8)t(8;11)(p23;?q23)t(11;17)(?q13;q11.2), t(8;14)(q24;q32), t(6;10)(q26;q23), der(11)t(11;16)(q13;q?11.2)dup(16)(q12q24), t(14;18)(q32;q21), del(16)(q21), der(17)t(4;17)(p11;q12)i(17)(q10); carries t(8;14) and t(14;18) effecting concurrent biallelic rearrangements of IGH with MYC and BCL2, respectively; resembles (unpublished) originator karyotype (by Siebert Lab, Kiel); heterozygosity of X-linked genes may indicate gender discrepancy regarding donor patient |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |