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| 产品名称： | TK-6 |
|---|---|
| 商品货号： | TS260196 |
| <strong>Cell line </strong>： | TK-6 |
| DSMZ no. ： | TS260196 |
| Species ： | human (Homo sapiens) |
| Cell type ： | chronic myeloid leukemia |
| Origin ： | established 1995 from the pleural effusion of a 30-year-old man with chronic myelogenous leukemia (CML) in T cell lineage blast crisis after bone marrow transplantation; cells carry the Philadelphia chromosome with a e14-a2 (b3-a2) BCR-ABL1 fusion gene. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 16450, 14484, 16449, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing singly in suspension; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; split saturated culture 1:2 every 3 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 60-72 hours |
| Harvest ： | maximum density at ca. 2.8 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD2 -, CD3 +, CD4 +, CD5 +, CD6 +, CD7 +, CD10 -, CD13 -, CD14 -, CD19 -, CD20 -, CD34 -, cyCD68 -, TCRalpha/beta -, TCRgamma/delta -, HLA-DR -; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype; 48(46-50)<2n>XY, +22, +22, del(1)(q24), del(6)(q21q24), dup(7)(p1?4q3?5)inv(7)(p14q35), t(9;22)(q34;q11), add(21)(p11), der(22)?inv(22)(p11q11)t(9;22)(q34;q11)x2; carries complex t(9;22) effecting BCR-ABL1 rearrangement with 3x genomic amplification in der(22); matches published karyotype |
| Molec. Genetics ： | expression of fusion gene BCR-ABL1 e14-a2 (b3-a2) confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |