微信小李
微信小章
产品简介
购买须知
| 产品名称： | MDA-MB-231 |
|---|---|
| 商品货号： | TS260199 |
| <strong>Cell line </strong>： | MDA-MB-231 |
| DSMZ no. ： | TS260199 |
| Species ： | human (Homo sapiens) |
| Cell type ： | breast carcinoma |
| Origin ： | established in 1973 from the pleural effusion of a 51-year-old woman with breast carcinoma after chemotherapy; described as ER-negative and tumorigenic in nude mice |
| Reference(s) ： | 14415, 17111, 17112 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial cells growing adherently as monolayer, sometimes forming spindle-like extensions; image; image |
| Medium ： | 90% Leibovitz´ L15 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5-1.0 x 106 cells/175 cm2; optimal split ratio 1:5 to 1:20 every 3-5 days using trypsin-EDTA |
| Incubation ： | at 37 °C, the originators suggest to incubate without gaseous exchange (lid of flask closed) |
| Doubling time ： | ca. 25-30 hours |
| Harvest ： | cell harvest of ca. 10-20 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |