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| 产品名称： | MIA-PACA-2 |
|---|---|
| 商品货号： | TS260200 |
| <strong>Cell line </strong>： | MIA-PACA-2 |
| DSMZ no. ： | TS260200 |
| Species ： | human (Homo sapiens) |
| Cell type ： | pancreas carcinoma |
| Origin ： | established in 1975 from the tumor tissue of a 65-year-old Caucasian man with undifferentiated pancreatic carcinoma |
| Reference(s) ： | 17196 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial cells growing adherently as monolayers and in small clumps; image; image |
| Medium ： | Dulbecco´s MEM + 10-20% h.i. FBS + 2.5% horse serum |
| Subculture ： | seed out at ca. 1 x 106 cells/80 cm2; split confluent culture 1:3 to 1:5 every 5 days using trypsin/EDTA; adherent clumps can be detached mechanically |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 30-40 hours |
| Harvest ： | cell harvest of ca. 15 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |