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| 产品名称： | OCI-AML4 |
|---|---|
| 商品货号： | TS260201 |
| <strong>Cell line </strong>： | OCI-AML4 |
| DSMZ no. ： | TS260201 |
| Species ： | human (Homo sapiens) |
| Cell type ： | acute myeloid leukemia |
| Origin ： | established in 1987 from the blast cells of the peripheral blood of a 35-year-old woman with acute myeloblastic leukemia (AML M4), secondary to the treatment of nodular sclerosing Hodgkin disease with radiation and chemo therapy; cells were described in the literature to grow slowly without stimulation but responds to IL-3, GM-CSF and mast cell growth factor (MGF); cells exhibit wild-type TP53 |
| Reference(s) ： | 16846, 16847 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round to polymorphic cells growing singly or in clumps in suspension, some cells are adherent; image; image; image |
| Medium ： | 80% alpha-MEM (with ribo- and deoxyribonucleosides) + 20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.5-1.5 x 106 cells/ml; optimal split ratio 1:2 to 1:4 every 2-4 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 24-30 hours |
| Harvest ： | maximum density at ca. 2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD4 +, CD13 +, CD14 -, CD15 -, CD33 +, HLA-DR +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | human flat moded near diploid karyotype with 3% polyploidy; -46(43-47)<2n>XX, der(6)(3qter->3q27::3q26->3q25::6p21->6q27::3q26->3q27::3q26->3qter), der(11)t(11;19)(q23;p13.3), der(16)t(1;16)(q22;p13), der(18)dup(18)(q11q23.2)t(X;18)(p22;q23.3), der(19)t(8;19)(q12;p13.1), der(22)t(11;22)(q23;q22)t(11;19)(q23;p13.3); carries semi-cryptic t(11;19) effecting rearrangement of KMT2A (MLL) with MLLT1 (ENL) |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |