微信小李
微信小章
产品简介
购买须知
| 产品名称： | MHH-TALL-2 |
|---|---|
| 商品货号： | TS260207 |
| <strong>Cell line </strong>： | MHH-TALL-2 |
| DSMZ no. ： | TS260207 |
| Species ： | human (Homo sapiens) |
| Cell type ： | T cell acute lymphoblastic leukemia |
| Origin ： | established in 1994 from the peripheral blood of a boy with T cell acute lymphoblastic leukemia (T-ALL) |
| Reference(s) ： | 16747 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single cells growing in suspension; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 1-1.5 x 106 cells/ml in 24-well plates; maintain at more than 1 x 106 cells/ml; split saturated culture 1:2 every 3 days; cells are difficult to culture; possibly no cell growth during the first week after thawing |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 60-72 hours |
| Harvest ： | maximum density at ca. 3 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD2 +, CD3 +, CD4 -, CD5 +, CD6 +, CD7 +, CD8 -, CD13 -, CD19 -, CD34 -, TCRalpha/beta +, TCRgamma/delta -; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics ： | human diploid karyotype with 2% polyploidy; 46(44-46)<2n>XY; no consistent abnormality detected |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |