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| 产品名称： | HT-93 |
|---|---|
| 商品货号： | TS260214 |
| <strong>Cell line </strong>： | HT-93 |
| DSMZ no. ： | TS260214 |
| Species ： | human (Homo sapiens) |
| Cell type ： | acute myeloid leukemia |
| Origin ： | established in 1993 from the peripheral blood of a 66-year-old man with acute promyelocytic leukemia (AML FAB M3) at relapse; described in the literature to carry t(15;17) and t(1;12) leading to PML-RARA and ETV6-ABL2 (ETV6-ARG) fusion genes, respectively |
| Reference(s) ： | 17286, 17287, 17298, 17299 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single cells growing in suspension; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out and maintain at ca. 0.5-1.0 x 106 cells/ml; optimal split ratio 1:2 every 2-3 days; culture should be started with 20% FBS in 24-well-plates |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | maximum density at ca. 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative PCR assay |
| Immunology ： | CD3 -, CD19 -, CD33 +, CD34 +, cyCD68 +, HLA-DR -; image |
| Fingerprint ： | genetic typing showed a pattern of short tandem repeats (STR) matching the reference profile of the international STR database |
| Cytogenetics ： | human near-diploid karyotype; 46(44-46)<2n>XY, t(1;12)(q25;p13), der(2)t(1;2)(q25;q31), der(4)t(1;4)(q21;q25)t(1;12)(q25;p13), der(6)del(6)(p23)t(4;6)(q25;q23), t(15;17)(q22;q21); carries complex t(1;12) and t(15;17) rearrangements effecting ETV6-ABL2 (ETV6-ARG) and PML-RARA rearrangements, respectively; resembles published karyotyp |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |