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| 产品名称： | SK-BR-3 |
|---|---|
| 商品货号： | TS260216 |
| <strong>Cell line </strong>： | SK-BR-3 |
| DSMZ no. ： | TS260216 |
| Species ： | human (Homo sapiens) |
| Cell type ： | breast carcinoma |
| Origin ： | established from the pleural effusion of a 43-year-old Caucasian woman with breast carcinoma in 1970 |
| Reference(s) ： | 17266 |
| Biosafety level ： | 1 |
| Risk assessment ： | The SK-BR-3 cell line is infected with bovine polyoma virus according to RNA-Seq data and PCR analsyses. The virus is categorized risk group 1 regarding humans and risk group 2 regarding animals according to German legislation |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent cells growing in monolayers changing from round shape after seeding to epithelial-like cells later on; image |
| Medium ： | 80% McCoy´s 5a + 20% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2 flask; split semi-confluent culture 1:3 to 1:5 once or twice a week using trypsin/EDTA for 5-10 min |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 2-3 days |
| Harvest ： | cell harvest of ca. 5 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -, BPyV |