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| 产品名称： | BALL-1 |
|---|---|
| 商品货号： | TS260220 |
| <strong>Cell line </strong>： | BALL-1 |
| DSMZ no. ： | TS260220 |
| Species ： | human (Homo sapiens) |
| Cell type ： | B cell leukemia |
| Origin ： | established in 1976 from the peripheral blood of a 75-year-old man with acute lymphoblastic leukemia (ALL) at diagnosis |
| Reference(s) ： | 14772, 19374 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single round cells growing in suspension; image; image ; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.2-2 x 106 cells/ml; optimal split ratio 1:2 - 1:5 every 2-5 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 48-72 hours |
| Harvest ： | maximum density at ca. 3 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | CD3 -, CD5 +, CD8 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, HLA-DR +, sm/cyIgG -, sm/cy IgM +, sm/cykappa -, sm/cylambda +; image |
| Fingerprint ： | genetic typing showed a short tandem repeat (STR) profile indicated as unique by a search of the international STR database |
| Cytogenetics ： | complex human near-pseudodiploid karyotype; 46(43-47)<2n>XY, der(1)t(1;3)(q32;q25) t(1;6)(p34;p21), der(3)t(1;3)(q32;q25)t(3;15)(p21;q21), der(4)t(4;18)(p11;q21)t(18;1)(q23;q21), der(6)del(6)(q23)t(2;6)(q31;p21), inv(6)(p22q25), t(8;14)(q24;q32)x1-2, del(9)(p22p23)der(9)t(3;9)(q11;p13), t(11;14)(q13;q32), der(11)t(8;11)(q22;q24), del(13)(q13q14), dup(13)(q21q31), der(14)t(14;15)(q32;q21), der(15)t(3;15)(q27;q21), der(15)t(8;15)(q24;q21), der(17)t(8;17)(q24;p11), der(18)qdp(18)(q12q21)t(4;18)(q22;p14), dup(19)(p11p13); sdl with der(14)t(11;14)x2; carries biallelic rearrangements of IGH with CCND1 and MYC, respectively, together with focal amplification of the BCL2/18q21 region which remains unrearranged; resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |