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| 产品名称： | 17.94 |
|---|---|
| 商品货号： | TS260223 |
| <strong>Cell line </strong>： | 17.94 |
| DSMZ no. ： | TS260223 |
| Species ： | human (Homo sapiens) |
| Cell type ： | Wilms´ tumor |
| Origin ： | established from the nephrectomy specimen of a large lobulated Wilms´ tumor of a 4-year-old girl; cells were described in the literature to express NCAM, SALL1, and CITED1, and to contain a TP53 mutation |
| Reference(s) ： | 18006 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | pleomorphic epithelioid cells growing adherently in monolayers; the cytoplasma of the cells exhibits numerous granules; image; image |
| Medium ： | 80% Dulbecco´s MEM + 20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5-1 x 106 cells/25 cm2 flask; split semi-confluent culture 1:2 once a week using TrypLE Express |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 1 week |
| Harvest ： | cell harvest of ca. 10 x 106 cells/25 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in PCR assay |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | genetic typing showed a pattern of short tandem repeats (STR) matching the reference profile of the international STR database |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV + |