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| 产品名称： | MDA-MB-468 |
|---|---|
| 商品货号： | TS260225 |
| <strong>Cell line </strong>： | MDA-MB-468 |
| DSMZ no. ： | TS260225 |
| Species ： | human (Homo sapiens) |
| Cell type ： | breast carcinoma |
| Origin ： | established in 1977 from the pleural effusion of a 51-year-old black woman with breast carcinoma; cells are described in the literature to carry a p53 point mutation at codon 273 leading to Arg-His substitution |
| Reference(s) ： | 14415, 17207, 15211 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial cells growing adherently as monolayer; image |
| Medium ： | 80% Leibovitz´ L15 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2 flask; split confluent culture 1:3 to 1:10 every 3-5 days using trypsin/EDTA |
| Incubation ： | at 37 °C, no exposure to CO2 (keep lid of flask closed) |
| Doubling time ： | ca. 30-40 hours |
| Harvest ： | cell harvest of ca. 15-20 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |