微信小李
微信小章
产品简介
购买须知
| 产品名称： | PACADD-159 |
|---|---|
| 商品货号： | TS260233 |
| <strong>Cell line </strong>： | PACADD-159 |
| DSMZ no. ： | TS260233 |
| Species ： | human (Homo sapiens) |
| Cell type ： | pancreas carcinoma |
| Origin ： | established in 2012 from a primary pancreatic tumor of a 78-year-old Caucasian man with ductal adenocarcinoma located at the tail of the pancreas (intermediate grade G2, pT3N1M0) |
| Reference(s) ： | 17618 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid cells growing adherently in monolayers; image; image |
| Medium ： | 80% mixture of DMEM and Keratinocyte SFM (at 1:1) + 20% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/25 cm2 flask; split confluent culture 1:2 once a week using trypsin/EDTA (wash cells twice with PBS before adding trypsin/EDTA and apply trypsin/EDTA for up to 20 min. to detach cells); cells may take 1-2 days to adhere again |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 1 week |
| Harvest ： | cell harvest of ca. 5 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | genetic typing showed a pattern of short tandem repeats (STR) matching the reference profile of the international STR database |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |